Abstract
The interaction of 1-anilinonaphthalene-8-sulfonic acid (ANS) and ethydium bromide with microsomal subfractions (rough, smooth I, smooth II) has been studied. Smooth microsomes have greater affinity for ANS and less affinity for ethydium bromide than rough microsomes. The neutralization of surface charge by metal cations increases membrane affinity for ANS. These findings are consistent with the hypothesis that different microsomal subfractions bear a different surface charge, smooth microsomes being less charged than rough microsomes. Metal cations also appear to induce a membrane structural change which results in an ANS and ethydium bromide fluorescence increase.
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