Abstract
AbstractHomology modeling involves taking a known sequence with an unknown structure and mapping it against a known structure of one or several similar (homologous) proteins. It would be expected that two proteins of similar origin and function would have reasonable structural similarity. Therefore it is possible to use the known structure as a template for modeling the structure of the unknown structure. Proteins that share the same function generally have similar structures. During alignment if two proteins show maximum sequence identity they also show a similar folding pattern. This principal has became the foundation of homology modeling.The Geminivirus taxonomic group of plant viruses is characterized by geminate particles and genomes consisting of single-stranded circular DNA molecules of about 2.5 to 2.8 kb in size. Agricultural plants are threatened by many diseases caused by whitefly-transmitted geminiviruses. Since these diseases are in a fast spreading phase, it is urgent to devise rapid diagnosis methods and to produce resistant plants.
Highlights
By Experiment we can predict protein structure, analysis of protein function, interaction, antigenic behavior and rational design of protein with increased stability
We find out the position of tyrosine with the help of Chimera, explain that there are difference in tyrosine position in Rep proteins of Tomato leaf curl Bangalore virus (TLCBV) and Tomato yellow leaf curl Thailand virus (TYLCTHV) because tyrosine play important role in DNA replication
Structure of unknown proteins can be identified on the basis of amino acid sequence pattern matching of both known and unknown proteins
Summary
By Experiment we can predict protein structure, analysis of protein function, interaction, antigenic behavior and rational design of protein with increased stability. Protein modeling are use to predict a structure from its sequence with an accuracy that allow users to use safely generated in silico protein models. Protein modeling is the only way to obtain structural information if experimental techniques fail. Geminiviruses are single - stranded DNA (ssDNA) viruses that cause severe disease in major crop plants worldwide. Most of geminiviruses belong to the genus Begomovirus, which are transmitted exclusively by the whitefly Bemisia tabaci (Harrison and Robinson, 1999). By using the homology modeling principles we can find the 3D structure of Rep protein of TLCBV and TYLCTHV and predict the stability of the protein
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