Abstract
Wheat is a propitious crop which renders several health benefits, but the modern wheat lacks essential micronutrients like zinc and iron. In this paper, various bioinformatics tools and molecular modeling approaches have been embodied for the development and exploration of structure and various properties, respectively. The NAM-B1 gene is a NAC transcription factor which plays an essential role in the translation machinery. It is also responsible for maintaining the nutritional seed grain quality in wheat. NAM-B1 gene is accountable for the Fe and Zn concentration in wheat and is also held responsible for delayed senescence in the crop by three weeks. In this study, we have developed the structural model of the NAM-B1 protein using the structure of 3ULX as template by Modeller 9.12. The resultant model was further processed and refined using various tools such as PROCHECK, ProSA, Verify3D and RMSD. As a result, the model developed was found to be conceivable with 62% a.a sequence identity with the template. Investigation revealed that conserved region found was accountable of the response generated during the improvement of the nutritional quality of grain. TaNAM-B1 plays an ingenious role in leaf senescence and abiotic stress tolerance in plants.
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