Abstract
Structural maintenance of chromosomes 4 (SMC-4) is a chromosomal ATPase which plays an important role in regulate chromosome assembly and segregation. However, the role of SMC-4 in the incidence of malignancies, especially colorectal cancer is still poorly understood. We here used quantitative PCR and Western blot analysis to examine SMC-4 mRNA and protein levels in primary colorectal cancer and paired normal colonic mucosa. SMC-4 clinicopathological significance was assessed by immunohistochemical staining in a tissue microarray (TMA) in which 118 cases of primary colorectal cancer were paired with noncancerous tissue. The biological function of SMC-4 knockdown was measured by CCK8 and plate colony formation assays. Fluorescence detection has been used to detect cell cycling and apoptosis. SMC-4 expression was significantly higher in colorectal cancer and associated with T stage, N stage, AJCC stage and differentiation. Knockdown of SMC-4 expression significantly suppressed the proliferation of cancer cells and degraded its malignant degree. Our clinical and experimental data suggest that SMC-4 may contribute to the progression of colorectal carcinogenesis. Our study provides a new therapeutic target for colorectal cancer treatment.
Highlights
Colorectal cancer (CRC) is one of the most frequent malignances in the world
Structural maintenance of chromosomes 4 (SMC-4) clinicopathological significance was assessed by immunohistochemical staining in a tissue microarray (TMA) in which 118 cases of primary colorectal cancer were paired with noncancerous tissue
Structural maintenance of chromosomes (SMC)-4 was up-regulated in colorectal cancerous tissue
Summary
Colorectal cancer (CRC) is one of the most frequent malignances in the world. It is the third most common cancer and the third leading cause of cancer death in the United State (Siegel et al, 2014). Throughout the articles relate to CRC in the journal ACJCP over the past two years, it is not difficult to find that the novel molecular markers with potential of treatment and prognosis have captured more and more attention (Chen et al, 2013; Zhu et al, 2013). Given this consideration, it is imperative to seek out a key gene associated with the progression and pathogenesis. Proteins is a member of the chromosomal ATPases family They are highly conserved from bacteria to human beings, and play critical roles in regulations of higher-order chromosome organization and dynamics (Losada and Hirano, 2005). The results suggested that SMC-4 might serve as a significant independent prognostic factor and potential target of CRC drug therapy
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