Abstract
A series of biodegradable triblock copolymers poly (ethylene glycol)-g-polyethylenimine-b-poly (dimethylaminoethyl l-glutamine) (PEG-g-PEI-b-PDMAEG) as novel vectors for gene therapy were synthesized and evaluated. Poly (ethylene glycol)-g-polyethylenimiene (PEG-g-PEI) was firstly obtained by linking of PEG and PEI using isophorone diisocyanate (IPDI) as coupling reagent. The anionic copolymerization of γ-benzyl l-glutamtae N-carboxyanhydride (BLG-NCA) using PEG-g-PEI as a macroinitiator was carried out, followed by aminolysis with 2-dimethylaminoethylamine to obtain the target water soluble triblock copolymer. The structures from PEG-g-PEI precursor to the triblock copolymers were confirmed by FT-IR. The particle sizes, zeta potentials of polyplexes were evaluated. All polyethylenimine derivates were revealed to compact plasmid DNA effectively to give polyplexes with suitable size (∼100 nm) and moderate ζ-potentials (10–15 mV) at N/P ratios of 30. The relationship between the composition of PEG-g-PEI-b-PDMAEG and the size, the ζ-potentials of corresponding copolymer/DNA complexes were also investigated.
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