Structural implications of basilar membrane compliance measurements.

Abstract

Static point-load measurements of basilar membrane compliance were made in the basal region of the excised guinea pig cochlea. Points on a radial line across the basilar membrane were displaced in one-half micron increments and the force required to maintain each increment recorded. The results are interpreted in terms of the material layers of the basilar membrane and displayed as compliance curves. In addition, a beam model of the basilar membrane, including the arches of Corti and the actual geometry of the pectinate zone, is constructed from anatomical data. The free parameters in this model are the modulus of elasticity of the transverse filaments and the effective spring stiffness of the arches. Compliance curves for the model are generated with a finite element approach and the parameters are obtained by requiring optimal agreement with the experimental measurements. The results show that the separation between fiber layers in the pectinate zone is relevant to the effective moment of inertia of the cross section and that the longitudinal coupling between the heads of the arches provides a rigidity to the arcuate zone not seen in the pectinate zone where longitudinal coupling is minimal. The elastic modulus calculated for the filaments is 1.8 GPa, approximately one-half that of keratin, while the cells and ground substance are five orders of magnitude softer.