Structural identification of alkyl glycosides obtained from the conversion of canna starch by immobilized α‐amylase from Aspergillus oryzae

Abstract

In this study, a novel route using immobilized α‐amylase from Aspergillus oryzae as a catalyst for the preparation of alkyl glycosides (AGlys) from canna starch and methanol in water has been demonstrated. The effects of the experimental conditions on the conversion yield of AGlys were investigated. The optimized conditions were a canna starch concentration of 100 g/L, an enzyme concentration of 82.8 U/mL, and a reaction time of 24 h. A crude yield of 2.01% of AGlys was obtained under the optimal experimental conditions corresponding to an in‐between situation of a donor‐limiting and an acceptor‐limiting regime. The products were then characterized by thin layer chromatography (TLC) and high performance liquid chromatography (HPLC). The results showed that methyl‐maltoside was the major product of the alcoholysis reaction.