Abstract
Genomic DNA from six euplasmic wheat-barley addition lines and from the wheat and barley parents were analyzed for the presence of alpha-amylase gene sequences, using plasmid DNAs from two different alpha-amylase cDNA clones as hybridization probes. Barley-specific alpha-amylase gene sequences were detected only in addition lines 1 and 6 carrying barley chromosomes 1 and 6, respectively. One cDNA clone probe hybridized strongly to sequences on chromosome 6 but not to those on chromosome 1. The other cDNA clone probe preferentially hybridized to sequences on chromosome 1. It is concluded that structural genes for alpha-amylases are located on barley chromosomes 1 and 6. alpha-Amylase genes on the same chromosome appear to be closely related to one another but substantially different from those on the other chromosome.
Highlights
Genomic DNA fromsix euplasmic wheat-barley addition lines and from the wheat and barley parents were analyzed for the presence of a-amylase gene sequences, using plasmid DNAs from two different aamylase cDNA clones as hybridization probes
Six euplasmic addition lines of Chinese Spring wheat each containing a pair of chromosomes from Betzes barley were constructed by Islam et al [8].A linecontaining barley chromosome 5 pair was not obtainedbecause of sterility
High-molecular-weight DNA was isolated from the leaves of thesixadditionlinesandthe two parents, digested with restriction enzymes, and the Southern blots were analyzed using nick-translated plasmiDdNA from a-amylaseclone 103 as the hybridization probe [5]
Summary
Genomic DNA fromsix euplasmic wheat-barley addition lines and from the wheat and barley parents were analyzed for the presence of a-amylase gene sequences, using plasmid DNAs from two different aamylase cDNA clones as hybridization probes. The EcoRI digest of Betzes barley DNA contains 4 DNA bands showing strong hybridization and one band showing weak hybridization (Fig. 2A, lane 1).
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