Abstract
Abstract Cell-wall material from ripe red beet was extracted as alcohol-insoluble residue (AIR). The CDTA-soluble extract from AIR was fractionated by anion-exchange chromatography. Four fractions were isolated by a step-wise increase in the ionic strength of the elution buffer. The main fraction was further fractionated by gel filtration chromatography. This chromatogram showed one regular broad peak, which was divided into three parts and pooled. All fractions isolated from both chromatographic systems were freeze-dried and their neutral sugar compositions as well as uronic acid contents were determined. Furthermore methylation analysis of these fractions were performed prior and after reduction of the pectic polysaccharides with NaBD 4 .
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