Abstract
During our biosynthesis study of cyanobacterial peptides including microcystins, we investigated the metabolic peptides in the hepatotoxic cyanobacteria, Anabaena sp. strains 90 and 202A2, in which the genetic analysis of the peptide synthetase had been carried out. For the exhaustive screening of cyanobacterial peptides, an analytical method using ESI-LC/MS on-line photodiode array detection was successfully developed and applied. Based on the analytical results, two groups of peptides, the cyclic depsipeptides having a 3-amino-6-hydroxy-2-piperidone moiety, anabaenopeptilides, and the cyclic peptides possessing an ureido linkage, anabaenopeptins, were isolated together with microcystins from both strains. Consequently, we confirmed the structures including the stereochemistry of the anabaenopeptilides encoded by the sequencing peptide synthetase genes in Anabaena strain 90.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.