Structural DNA and genetically active DNA in dinoflagellate chromosomes

Abstract

High resolution electron microscope autoradiographs of [ 3H]adenine incorporation in the dinoflagellate Prorocentrum micans suggest that RNA transcription occurs on extrachromosomal DNA filaments, but not on DNA in the main body of the chromosome. This genetically inactive DNA has an important role, however, in stabilising chromosome structure by its association with protein matrix. Evidence for the importance of this molecular association (which is probably cation-mediated) is provided by alkaline buffer extraction of the protein matrix in chromosomes of Amphidinium carterae, leading to complete destabilisation of the DNA framework. The clear distinction between structural DNA and genetically active DNA in these chromosomes provides a marked contrast to normal eucaryote chromosomes. This distinction is related to the occurrence of high DNA values in these organisms, and the evolutionary status of the dinoflagellate chromosome.