Structural differences in the nucleic acids of some tobacco mosaic virus strains: I. Monopyrimidine nucleotides in ribonuclease digests

Abstract

The nucleic acids obtained from four strains of tobacco mosaic virus were digested with crystalline pancreatic ribonuclease at 23° for 12 hours at pH 7.6. The monopyrimidine nucleotides, uridylic acid and cytidylic acid, were isolated from such digests and estimated. Significantly greater quantities of pyrimidine mononucleotides were released from the nucleic acid of M strain than from those of strains, TMV, HR and YA. From this result it was concluded that the intramolecular distribution of pyrimidine nucleotides in the nucleic acid of M strain differs from those of strains, TMV, HR and YA.