Abstract
Edwardsiella tarda, a Gram-negative bacterium, is an important cause of hemorrhagic septicemia in fish and also of gastro- and extraintestinal infections in humans. The lipopolysaccharide produced by the fish pathogenic strain E. tarda MT 108 was isolated and the structure of its antigenic O-polysaccharide component determined by the application of chemical analyses, high-resolution 1D and 2D nuclear magnetic resonance spectroscopy, and mass spectrometry. The polysaccharide was found to be a polymer of a repeating pentasaccharide unit composed of 2-acetamido-2-deoxy- d-glucose ( d-GlcNAc), 2-acetamido-2-deoxy- d-galactose ( d-GalNAc), d-galactose ( d-Gal), l-rhamnose ( l-Rha), d-galacturonic acid ( d-GalA) and (2 S,3 R)-threonine (1:1:1:1:1:1) having the structure: ▪
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