Abstract
The O-chain polysaccharide produced by a mild acid degradation of Aeromonas caviae ATCC 15468 lipopolysaccharide was found to be composed of l-rhamnose, 2-acetamido-2-deoxy- d-glucose, 2-acetamido-2-deoxy- d-galactose and phosphoglycerol. Subsequent methylation and CE–ESIMS analyses and 1D/2D NMR ( 1H, 13C and 31P) spectroscopy showed that the O-chain polysaccharide is a high-molecular-mass acidic branched polymer of tetrasaccharide repeating units with a phosphoglycerol substituent having the following structure: [Display omitted] where Gro represents glycerol and P represents a phosphate group.
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