Abstract
Epichlorohydrin (ECH) is a bifunctional alkylating agent used extensively as an industrial intermediate, a laboratory reagent, and as an insecticide. ECH is classified as a probable human carcinogen since occupational exposure to ECH has been linked to elevated cancer risk for industrial workers. The ability of ECH to form DNA‐DNA cross‐links by consecutive alkylation of two nucleobases within a DNA molecule leads to the possibility of both cytotoxic and promutagenic properties. Previous work provided evidence for the formation of interstrand ECH‐DEB cross‐links involving deoxyguanosine residues within 5′‐GC and 5′‐GGC sites. However, the covalent structure of ECH‐induced DNA cross‐links has not been determined. In the present study, ECH interstrand cross‐links within synthetic DNA oligonucleotides containing a central 5′‐GGC site will be purified and characterized via electrospray ionization mass spectroscopy. This project was supported by grants from the National Center for Research Resources (5P20RR016463–12) and the National Institute of General Medical Sciences (8 P20 GM103423–12) from the National Institutes of Health.
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