Abstract
Dendrobium officinale is a widely used medicinal plant in China with numerous bio-activities. However, the main structure and anti-tumor activity of the polysaccharides from this plant have not been investigated. In this study, we elucidated the main structure of polysaccharides purified with DEAE and Sephadex G-25 from Dendrobium officinale grown under different planting conditions. In addition, the anti-tumor activity was tested via MTT assays. The results showed that the polysaccharides of Dendrobium officinale grown under different conditions were almost the same, with slight differences in the branched chain; both polysaccharide fractions consisted of (1→4)-linked mannose and (1→4)-linked glucose, with an O-acetyl group in the mannose. After degradation, the polysaccharide fractions from wild plants showed significant anti-proliferation activity in HeLa cells. The fractions F1 and F3 induced apoptosis by up-regulating the expression of ERK, JNK, and p38. We concluded that polysaccharides from Dendrobium officinale planted in the wild exhibit significant anti-tumor effects only after being degraded to smaller molecular weight species. The planting mode is a significant factor in the pharmacological activity of Dendrobium officinale. We advise that the planting conditions for Dendrobium officinale should be changed.
Highlights
Dendrobium officinale is a medicinal plant that has been widely used in China for thousands of years.As the medicinal materials market has grown, the demand for the medicinal plant Dendrobium officinale has increased dramatically [1]
G-25 column, the Purified Polysaccharide Fractions (PPFs) from the two sources of Dendrobium officinale were divided into two groups: the first group was named DWDOP and the second, FWDOP
The plant Dendrobium officinale has been used for thousands of years in China as a folk medicine
Summary
Dendrobium officinale is a medicinal plant that has been widely used in China for thousands of years. As the medicinal materials market has grown, the demand for the medicinal plant Dendrobium officinale has increased dramatically [1]. Since the wild resource is limited, researchers have developed a series of greenhouse cultivation methods to improve productivity. The metabolic components in plants are influenced greatly by the environment. Plants planted in the greenhouse are different from those grown in the wild. The external properties of this medicinal plant planted in the wild and cultivated in the greenhouse are different. No studies have reported pharmacological differences between these two planting modes.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
