Abstract
An insoluble polysaccharide with immunostimulating activity, which we have designated as pichilan, was isolated from the cell wall of Pichia fermentans (CBS 187) by repeated treatment with hot alkali and hot acetic acid. Partial hydrolysis led to the liberation of laminariosides and gentiobiose. A study of the products obtained by methylation, Smith degradation and endoglucanase hydrolysis showed that the polysaccharide is a moderately branched, (1→3)-β-D-glucan with a high molecular weight (approximately 200000) containing 10% β-(1→6)-linked glucopyranosyl residues and two different types of branches, a single glucosyl group and a long glucosyl chain.
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