Abstract
An edible fungal polysaccharide termed as ABP was obtained by extraction with hot water, and followed successive chromatographic purification using DEAE-Sepharose Fast Flow column and Sephacryl S-300 High-Resolution column. A symmetrical peak was obtained on high-performance size-exclusion chromatography with an average molecular weight of 5.17 × 104 Da, which was named ABP, and its main components were d-glucose and d-mannose. Based on the study of methylation analysis, along with FT-IR, GC, GC-MS, 1D 1H and 13C NMR and 2D NMR (H-HCOSY, TOCSY, HMQC, and NOESY), its chemical structure was featured with a repeating unit (1→6) linking β-d-Glcp as the main backbone with (1→4)-linked α-d-Manp units. The structure of the mainly repeating units of ABP was established as: →6)-β-D-Glucp-(1→4)-α-D-Manp(1→6)-β-D-Glucp-(1→6)-β-D-Glucp-(1→
Highlights
For thousands of years, mushrooms have been known as a source of medicine [1], many studies showed that polysaccharides were the main occurring biological constituents in common mushrooms.Int
As part of a continuing investigation of the chemical structures and biological activities of the polysaccharides from this fungus, in this manuscript, we report the isolation and structural characterization of A. bisporus polysaccharide (ABP) from A. bisporus
The water-soluble polysaccharide from the fruiting bodies of A. bisporus was purified by DEAE-Sepharose Fast Flow column and gel-permeation chromatography on Sephacryl S-300
Summary
Mushrooms have been known as a source of medicine [1], many studies showed that polysaccharides were the main occurring biological constituents in common mushrooms.Int. Many evidences have indicated that the biological activities of polysaccharides depended on their structural features such as the compositions of sugar, type of glycosyl linkage, branch structures, molecular weight and concentration [7,8,9]. In this field, the β-(1→3), (1→6) glycosidic linkages are supposed to play an important role in enhancing the antitumor and the immunomodulatory effects [10,11,12,13,14]
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