STRUCTURAL CHARACTERIZATION, BIOLOGICAL EVALUATION AND MOLECULAR DOCKING STUDIES OF FUCOIDAN ISOLATED FROM BROWN MARINE ALGAE

Abstract

The objective of this present investigation was to evaluate the hemolytic, antiinflammatory, antioxidant, antiproliferative activity and molecular docking studies of fucoidan isolated from brown seaweed Sargassum wightii. Fucodian was isolated from brown seaweed of S.wightii using ethanol precipitation. Functional groups and structural characterization of fucodian was analyzed by Fourier transform infrared spectroscopy (FT-IR) and nuclear magnetic resonance spectroscopy, Morphology of the isolated fucoidan was performed by scanning electron microscopy (SEM). The antioxidant properties were determined by DPPH scavenging, nitric oxide scavenging and reducing power assays. The biocompatibility of fucodian was assayed by hemolysis and anti- inflammatory studies and the in vitro antiproliferative activity was assayed by 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) against human lymphoma (U 937) cell lines. The maximum DPPH activity (79.92± 0.35), NO scavenging activity (51±0.28%), reducing ability (230± 0.87 in terms of FRAP values) was found at the concentration of 100μg/mL Fucoidan was potent against hemolysis of the erythrocyte in a concentration dependent manner suggesting non- toxic nature. The antiproliferative effect of fucoidan showed a higher percentage of inhibition, that is 50% of the cell death after 48h of incubation was achieved in a dose dependent manner. The fucoidan has revealed a significant biding interaction against human kinase (CK2 formerly known as casein kinase 2) as target protein to uphold antioxidant and anti proliferative activities. These findings indicated that the isolated fucoidan from S.wightii found to be a promising candidate for the development of an anti cancer compound for drug delivery applications as well as for further investigations in various cell lines.