Structural characterization and pro-angiogenic property of a polysaccharide isolated from red seaweed Bangia fusco-purpurea

Abstract

In this study, we evaluated the structural characteristics and novel biological activity of polysaccharide purified from red seaweed Bangia fusco-purpurea (BFP). Methylation, GC/MS, and NMR analyses suggested that the proposal repeating structure of BFP was →3)-β-D-Galp-(1→, →3)-β-D-Galp6S-(1 → 4)-α-D-Galp-(1→, →4)-α-D-Galp-(1 → 4)-α-L-AnGalp-(1 → 3)-β-D-Galp-(1→, and →4)-α-D-Galp-(1 → at a molar ratio of 13: 1: 1: 1. Interestingly, BFP exhibited significant cell migration- and tube formation-promoting activities toward human umbilical vein endothelial cells (HUVECs) in a concentration-dependent manner via increasing the N-cadherin expression and decreasing the E-cadherin expression. Furthermore, ERK and p38 mitogen-activated protein kinase (MAPK) specific inhibitors exhibited potent inhibitory effects on BFP-induced cell migration but not JNK MAPK inhibitor, suggesting ERK and p38 MAPK signaling pathways were mainly involved in BFP-induced cell migration. Moreover, vascular endothelial growth factor (VEGF) receptor tyrosine kinase inhibitor significantly inhibited BFP-induced cell migration and tube formation in HUVECs, suggesting VEGF receptors of HUVECs were involved in the pro-angiogenesis activity of BFP. This is the first report that a sulfated polysaccharide possessing a pro-angiogenic effect was obtained from red seaweed. Our findings are expected to promote the practical use of red seaweed B. fusco-purpurea and its polysaccharide in the development of the in vitro and ex vivo vascular endothelial cell-based cell therapy products.