Structural Characterization and Functional Interaction Analysis of Radiation Induced Protein Flt3 in Human and Mouse

Abstract

Increasing risk of radiation exposure can be due to various reasons such as terrorist attacks, detonation of nuclear reactors, nuclear weapons, as well as acute radiation exposure on large scale poses high risk to humankind. Therefore, there is a critical need of identification and authentication of fast and early-response radiation induced harm biomarkers to distinguish between exposed and non-exposed individuals on a large-scale basis. This study deals with structural characterization of radiation induced proteins that are probable biomarkers such as Flt3L using database tools such as prot-param for physio-chemical properties, GOR IV to predict the secondary structure, swiss modelling for 3D model of protein, stitch and string analysis for protein-chemical interaction and protein-protein interaction respectively. In-silico analysis showcasing, protein Flt3L physical parameters such as theoretical PI 8.72, hydrophobicity 0.100, aliphatic index 111.81. Secondary structure, alpha helix 41.38%, extended strand 11.64%. Homology modelling showing sequence identity 74.26%, coverage 0.50 and range 1-101. In stich, Flt3 activating KITLG and showing binding affinity to Zn molecule. Identification of protein-protein interacting functional partner of Flt3L using string shows interactions with polyubiquitin-C (UBC), KITLG, Nras proto onco genes, etc, it showed experimental evidence of Flt3L and KITLG protein. Hence, by using in-silico analysis we can perform a preliminary study to predict the structures and chemical interaction of potential radiation induced probable biomarkers that can be utilized for rapid detection of radiation exposure and drugdiscovery.