Abstract
Abstract The structural characterization and bioactivities of a polysaccharide from the stalk residue of Pleurotus eryngii were investigated. Firstly the stalk residue of P. eryngii was collected from the top portion of spent mushroom substrate and processed to yield water-soluble polysaccharide. Subsequently this crude polysaccharide was purified by DEAE Sepharose CL-6B ion exchange chromatography and Sepharose CL-6B size-exclusion chromatography. Then its structural features were investigated by gas chromatography (GC), gel permeation chromatography (GPC), methylation analysis, and Fourier Transform Infrared Spectrum (FT-IR). The results showed that it was heteropolysaccharide and mainly composed of glucose (82.4%). The backbone of P-2a mainly consisted of 1, 3-linked (42.7%) and 1, 6-linked (35.5%) glucose residues. Besides, in vitro antioxidant assay showed that P-2a exerted a high scavenging effects on hydroxyl radical and in vitro antitumor assay showed it had a dose-dependent antiproliferative effect against human gastric MGC-803 cancer cells & human epithelial Hela cancer cells. The findings of this study suggested that the polysaccharide extracted from the stalk residue has the similar structure and bioactivities as that from fruit-body of the mushroom. It could be potentially used as a natural source for the development of health-care food.
Highlights
Mushroom cultivation is a promising cottage industry in China
Two major elution peaks named as P-1 and P-2 were separated by DEAE-Sepharose CL-6B anion-exchange chromatography according to their ionic-binding capacity (Figure 1A)
The first peak (P-1) mainly contained a neutral polysaccharide as it was gained by elution with distilled water, while the second peak (P-2) was mainly ascribed to the acidic polysaccharide because it was collected by elution with 0.1 mol/L NaCl solutions
Summary
Mushroom cultivation is a promising cottage industry in China. It supplies delicious and nutritious foods (Zheng et al 2018). Mushroom cultivation is beneficial for the ecological cycle of agriculture because the raw materials for raising mushroom are recyclable cereal straw and organic waste or other organic by-products. These huge lignocelluloses biomass wastes can be conversed into human food by means of cropping mushrooms (Zhang et al, 2014). Mushroom production can provide good income opportunities for families who do not have enough land to produce crops and raise animals (Banasik et al, 2017)
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