Structural characterization and anti-oxidant activity of polysaccharide HVP-1 from Volvariella volvacea

Abstract

In this study, a novel homogeneous polysaccharide (HVP-1) was purified from the Volvariella volvacea. Its structural characteristics and anti-oxidant activity in vitro were further evaluated. The results revealed that HVP-1 was composed of mannose, glucose, galactose and arabinose in a molar ratio (mol %) of 55.37: 15.74: 25.20: 3.69. Its main chain consisted of →4)-β-D-Galp-(1→, →6)-α-D-Glcp-(1→, →3)-α-D-Glcp-(1→, →4)-β-D-Manp-(1→ and →3,6)-β-D-Manp-(1→. The branched structure α-L-Araf-(1→, →2)-β-D-Glcp-(1→ and →6)-β-D-Manp-(1→ were connected to →3,6)-β-D-Manp-(1→ through the O-3 position. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) showed that HVP-1 had porous sheet-like structure with a triple helix conformation. Anti-oxidant activity experiments showed that HVP-1 alleviated H2O2-induced oxidative damage by reducing the accumulation of reactive oxygen species, increasing the activity of related enzymes in cells, and activating the Nrf2/HO-1 signaling pathway. These results suggested that HVP-1 had the potential to be used as a natural anti-oxidant in functional foods and pharmaceuticals.