Abstract
AbstractA method was developed for rapid qualitative determination of lignocellulose in the tobacco cell wall by utilizing 2D heteronuclear single quantum coherence NMR spectra (2D HSQC NMR). Traditional methods for analyzing the structure of lignocellulose involve many steps of separation and extraction, which is labor-intensive. In this work, the whole cell wall was milled and dissolved in deuterium solvent. The solvent dimethylsulfoxide (DMSO-d6) containing hexamethylphosphoramide (HMPA-d18) enhanced swelling of the sample and gave high-resolution spectra. The tobacco samples are ball milled at different ball milling times, and the state of the particles is observed through an electron microscope, and then the probability of the particles being less than 5 µm is counted. Through the comparison of the abundance and integration of the peak signals in the spectra under different transmittances, it was determined that when the milling time was 6 h, the quality of the NMR spectra was the best. The optimum conditions of characterizing tobacco structure were DMSO-d6/HMPA-d18solution and 6 h milling time. Under these conditions, complete representation of the structure of lignocellulose and simplified process could be achieved.
Highlights
Plant cell walls, known as lignocellulosic biomass, are mainly composed of cellulose, hemicellulose, and lignin [1]
DMSO-d6/HMPA-d18 was selected as the solvent to dissolve plant cell wall materials in this study
DMSO-d6/HMPA-d18 was selected as the solvent for dissolving lignocellulose
Summary
Known as lignocellulosic biomass, are mainly composed of cellulose, hemicellulose, and lignin [1]. Cellulose is a linear macromolecular compound formed by the linkage of 1,4-glucoside [2]. Hemicelluloses is a class of highly branched polysaccharides and is a heterogeneous glycan composed of heteropolysaccharides containing different pentoses and hexoses [3]. Lignin is a phenolic polymer formed from phenylpropane units through aryl ether bonds and carbon–carbon bonds, consisting of three lignin units called p-hydroxyphenyl (H), guaiacyl (G), and syringyl (S) [4]. Hemicellulose and lignin in plant cell walls form a network structure through covalent bonds, and cellulose is embedded in the network and makes the lignocellulose form a tight interaction [5,6]. In the field of tobacco, the structural changes of lignocellulose will cause the changes of tobacco properties during the process of tobacco production and transformation, so characterizing structural state of tobacco fast will provide guidance for the reduction of tobacco damage [7]
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