Abstract
The structure of the native dextran produced by Streptococcus sanguis ATCC 10558 was analyzed by g.l.c.-m.s. of the methylated alditol acetates derived from the polymer. The results indicate that the polymer contains d-glucosyl residues substituted at C-6 or C-3, or both, as well as unsubstituted d-glucosyl residues. These data are correlated with the proportion of C-6 and non-C-6 substitution as measured by p.m.r. Similar data are reported for the analysis performed on a dextran produced by action of a partially purified dextransucrase on sucrose. The proportion of d-glucosyl residues substituted at C-3 is diminished in this case. It is concluded that several enzymes are involved in the dextran synthesis.
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