Abstract
An alkali-extractable water-soluble polysaccharide, Pc-4, was isolated from the lichen Peltigera canina using ethanol fractionation and size exclusion chromatography. The average molecular weight of Pc-4 was estimated to be 53 kDa. Structural characterisation of Pc-4 was performed by methanolysis, methylation analysis and NMR-spectroscopy (1D proton, COSY, NOESY, TOCSY and HSQC). According to data obtained, Pc-4 is a highly branched galactomannan, with (1→6)-linked α-mannopyranosyl units in the main chain, which are mainly disubstituted at O2 and O4 by single unsubstituted units of α-Man p and β-Gal p, respectively. Pc-4 resembles the lichen galactomannans described previously; however, Pc-4 has higher degree of branching.
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