Abstract
In this study Bacillus amyloliquefaciens RK3 was isolated from a sugar mill effluent-contaminated soil and utilised to generate a potential polysaccharide with anti-Alzheimer’s activity. Traditional and molecular methods were used to validate the strain. The polysaccharide produced by B. amyloliquefaciens RK3 was purified, and the yield was estimated to be 10.35 gL−1. Following purification, the polysaccharide was structurally and chemically analysed. The structural analysis revealed the polysaccharide consists of α-d-mannopyranose (α-d-Manp) and β-d-galactopyranose (β-d-Galp) monosaccharide units connected through glycosidic linkages (i.e., β-d-Galp(1→6)β-d-Galp (1→6)β-d-Galp(1→2)β-d-Galp(1→2)[β-d-Galp(1→6)]β-d-Galp(1→2)α-d-Manp(1→6)α-d-Manp (1→6)α-d-Manp(1→6)α-d-Manp(1→6)α-d-Manp). The scanning electron microscopy and energy-dispersive X-ray spectroscopy imaging of polysaccharides emphasise their compactness and branching in the usual tubular heteropolysaccharide structure. The purified exopolysaccharide significantly impacted the plaques formed by the amyloid proteins during Alzheimer’s disease. Further, the results also highlighted the potential applicability of exopolysaccharide in various industrial and pharmaceutical applications.
Highlights
Microorganisms produce exopolysaccharides (EPS) by utilising different nutrient sources
The soil was dried in the oven at 30 ◦ C to remove the moisture content
The EPS production nature of the strain RK3 was initially confirmed on sucrose supplemented basal media [52,53]
Summary
Microorganisms produce exopolysaccharides (EPS) by utilising different nutrient sources. EPS are ubiquitous and have been reported from diverse sources of microorganisms [1]. The different EPS are functionally characterised as significant polymeric substances, which are extracellularly produced by various microbial species [2]. EPS can execute different essential biological functions in various organisms. Microbial EPSs are known to exhibit antioxidant properties, anti-ulcer and anti-toxin activity [3]. They are classified into homopolysaccharides with only one or the same type of sugar molecules, e.g., cellulose, alternan, pullulan, mutant, levan, dextran and curdlan. The structure of the microbial EPS is mainly composed of monomer units linked by glycosidic linkages [6]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
