Abstract

Endosperm transfer cells mainly occur in the epithelial layer of the endosperm and transport the nutrient unloaded by the maternal vascular tissue. They have wall ingrowths that can facilitate solute transportation. Here we report our further investigation of endosperm transfer cells in sorghum (Sorghum bicolor L. Moench). We observed endosperm transfer cells, embryo, and endosperm with different kinds of microscopes. Our experimental results showed that the distribution and configuration of endosperm transfer cells were fit for solute transportation, and they had a tight relationship with the embryo and endosperm.

Highlights

  • Observation of endosperm transfer cells under light and transmission electron microscopes The observed region was cut into pieces of suitable size and fixed in 3% glutaraldehyde for 3 h or more

  • The endosperm epithelial cells consisted of aleurone cells and ETC.[9]

  • Transfer cells (TC) have been found in many grain cary- blue, and observed under the light microscope 1C)

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Summary

Introduction

Observation of endosperm transfer cells under light and transmission electron microscopes The observed region was cut into pieces of suitable size and fixed in 3% glutaraldehyde for 3 h or more. The endosperm epithelial cells consisted of aleurone cells and ETC.[9] At 6 days after pollin ation (DAP), radially the ETC lie near the maternal tissues, which comprise nucellar cells, placentochalazal cells, and vascular bundles from the inner to the outer layers (Figure 1B). At 9 DAP, nucellar cells adjacent to the ETC disappear and the endosperm cavity forms

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