STRUCTURAL CHANGES OF THE COLON WALL IN CASE OF DEVELOPMENT OF ENTERAL INSUFFICIENCY CAUSED BY EXPERIMENTAL PERITONITIS

Abstract

Objective: to study structural changes of the colon wall and development of enteral dysfunction in an experimental course of acute peritonitis. Materials and Methods. Experimental studies were conducted on 21 mature albino nonlinear rats. Under thiopental anaesthesia peritonitis was caused by puncture of the ascending colon and the introduction of freshly prepared 15% solution in the dose of 0.5 ml per 100 g of the body weight of a rat. On the 1st, 3rd and 7th day of experimental peritonitis animals were decapitated under calypsol anesthesia. The pieces of the small intestine were taken for histological examination determining changes histologically. Results. Structural changes were found to occur on the first, third, and 7 day of experimental peritonitis in the lining of the colon and continued to grow over time, manifested by its consolidation at the expense of pronounced lymph and histiocytic infiltration and combined focal leukocyte infiltration, corresponding to morphological structure of colitis. Lumen of crypts is reduced at the expense of an increased activity of secretory epithelial cells and degenerative changes. Uneven blood supply of the vessels combined with perivascular edema is found in the subepithelial layer. Conclusion. Increased secretory activity and development of degenerative changes in the superficial epithelium occur in the colon mucosa during experimental peritonitis. Epithelial degenerative changes with pronounced histiocytic and lymph infiltration of the stroma and its edema increase from 3 to 7 days.