Abstract
The work is devoted to the study of structural changes of enterocytes in the mucous membrane of the duodenum in a burn injury of the skin of a rat under conditions of experimental streptozotocin induced diabetes. The study was carried out on laboratory white adult rats-males weighing 180-210 g. The control group consisted of 21 animals without somatic pathology, the first experimental group consisted of 21 rats with skin burn injury, the second experimental group cleared 21 rats with skin burn and experimental streptozotocin induced diabetes. A model of experimental diabetes mellitus was reproduced by administering streptozotocin intraperitoneally once in dose of 50 mg/kg to rats. In an experimental simulation of a skin burn, two copper plates in the form of an ellipse were kept in water at 100oC for 10 minutes and, under ether anesthesia conditions, were applied simultaneously symmetrically on both exposed parts of the body of rats with an exposure for 10 seconds. Burn skin damage in rats was II-AB degrees – dermal surface burn (according to the old classification III-A degree) with a total area of 21-23% of the body surface with the development of burn shock. For morphological studies, the duodenum was taken, fragments of which were processed using conventional light and electron microscopy. The main criteria for assessing damage the enterocytes of the duodenal mucosa were the results studies of histological and ultrastructural data over 7, 14 and 21 days after a skin burn. The results of the studies showed that the damage of the enterocytes of the duodenal mucosa is based on deep destructive changes, which after 21 days (at the stage of septic toxemia), as a rule, are not reversible and develop against the background of significant intoxication of the organism. In the mucous membrane of the duodenum with burn injury of the skin associated with diabetes mellitus, there is a deterioration of the manifestations of the adaptive response and prolongation of destructive processes, accompanied by a violation of intercellular interactions in cytoarchitectically modified and deformed villi and crypts.
Highlights
In modern conditions of intensive industrialization and more increasing growing worldwide use of heat sources in everyday life, there is an increase in the frequency of burn traumatic injuries [10, 12, 13, 14, 18, 20]
The main criteria for assessing damage the enterocytes of the duodenal mucosa were the results studies of histological and ultrastructural data over 7, 14 and 21 days after a skin burn
The results of the studies showed that the base of damage to the duodenal mucosa enterocytes consist of deep destructive changes, which after 21 days, as a rule, are not reverse and develop on the background of significant intoxication of the body
Summary
In modern conditions of intensive industrialization and more increasing growing worldwide use of heat sources in everyday life, there is an increase in the frequency of burn traumatic injuries [10, 12, 13, 14, 18, 20]. Deep widespread burns are characterized by damage to the integumentary tissues, and cause burn disease, which is characterized by different, lasting and peculiar general morphological and functional changes of all organs and systems of the organism [16]. Membrane duodenum the conditions of association burn disease with diabetes have so far remained unheeded by researchers. On this basis, the important role of duodenal dysfunction in the pathogenesis of burn disease [4, 6] determines the need to study the morphogenesis of destructive and regenerative processes in its mucous membrane at different times after burn injury under the conditions of the course of diabetes mellitus
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