Abstract
In this work, surface-enhanced Raman spectra of ten genomic DNAs extracted from leaf tissues of different grapevine (Vitis vinifera L.) varieties, respectively, are analyzed in the wavenumber range 300–1800 cm−1. Furthermore, structural changes induced in grapevine genomic nucleic acids upon femtosecond (170 fs) infrared (IR) laser pulse irradiation (λ = 1100 nm) are discussed in detail for seven genomic DNAs, respectively. Surface-enhanced Raman spectroscopy (SERS) signatures, vibrational band assignments and structural characterization of genomic DNAs are reported for each case. As a general observation, the wavenumber range between 1500 and 1660 cm−1 of the spectra seems to be modified upon laser treatment. This finding could reflect changes in the base-stacking interactions in DNA. Spectral shifts are mainly attributed to purines (dA, dG) and deoxyribose. Pyrimidine residues seem to be less affected by IR femtosecond laser pulse irradiation. Furthermore, changes in the conformational properties of nucleic acid segments are observed after laser treatment. We have found that DNA isolated from Feteasca Neagra grapevine leaf tissues is the most structurally-responsive system to the femtosecond IR laser irradiation process. In addition, using unbiased computational resources by means of principal component analysis (PCA), eight different grapevine varieties were discriminated.
Highlights
Nowadays, it is established that DNA is one of the principal macromolecular targets for radiation damage
We have described in detail elsewhere the method used for the preparation of Ag colloidal SERS
Surface-Enhanced Raman Spectroscopy of DNA Extracted from Grapevine Leaf Tissues
Summary
It is established that DNA is one of the principal macromolecular targets for radiation damage. Any agent that causes DNA damage has potential applications in cancer therapy, since cancer is a disease of DNA, caused mainly by alterations of the genome. Femtosecond laser technology offers potential new insights that could be used in this research. Molecular mechanisms of the biological action of femtosecond IR pulsed irradiation on DNA have not yet been completely established. It was found that DNA strand breaks are induced via nonlinear excitation with femtosecond laser pulses at λ = 1050 nm [1]. The selective photo-disruption of the nucleus of a single living cell, without modifying the cell morphology, is demonstrated in living human breast cells using near-infrared (NIR) (790 nm) femtosecond (110 fs)
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