Abstract
SummaryG3PDH was isolated from rainbow trout acclimated to 15 and 5°. Anti-sera to each of the enzymes were prepared in rabbits. Microprecipitin tests showed that the short-term (ST) antiserum reacted only with its specific antigen. However, with the long-term (LT) antisera, there was some cross reaction. Ouchterlony double diffusion confirmed these results. Kinetic studies of each of the G3PDH enzymes in the presence of its specific antiserum were carried out. Specific ST and LT antisera significantly inhibited the activity of the enzyme. No significant effect was seen with the antisera to the noncomplementary enzyme. Some cross reaction was seen with the LT antisera in both instances. The Lineweaver-Burke analyses demonstrated no change in the Km while the Vmax was reduced, indicative of noncompetitive inhibition by the specific antisera.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
