Abstract
Bridging integrator-1 (BIN1) gene is associated with an increased risk to develop Alzheimer’s disease, a tauopathy characterized by intra-neuronal accumulation of phosphorylated Tau protein as paired helical filaments. Direct interaction of BIN1 and Tau proteins was demonstrated to be mediated through BIN1 SH3 C-terminal domain and Tau (210–240) peptide within Tau proline-rich domain. We previously showed that BIN1 SH3 interaction with Tau is decreased by phosphorylation within Tau proline-rich domain, of at least T231. In addition, the BIN1/Tau interaction is characterized by a dynamic equilibrium between a closed and open conformations of BIN1 isoform 1, involving an intramolecular interaction with its C-terminal BIN1 SH3 domain. However, the role of the BIN1/Tau interaction, and its potential dysregulation in Alzheimer’s disease, is not yet fully understood. Here we showed that within Tau (210–240) peptide, among the two proline-rich motifs potentially recognized by SH3 domains, only motif P216TPPTR221 is bound by BIN1 SH3. A structural model of the complex between BIN1 SH3 and Tau peptide (213–229), based on nuclear magnetic resonance spectroscopy data, revealed the molecular detail of the interaction. P216 and P219 within the proline-rich motif were in direct contact with the aromatic F588 and W562 of the BIN1 SH3 domain. The contact surface is extended through electrostatic interactions between the positively charged R221 and K224 residues of Tau peptide and those negatively charged of BIN1 SH3, corresponding to E556 and E557. We next investigated the impact of multiple Tau phosphorylations within Tau (210–240) on its interaction with BIN1 isoform 1. Tau (210–240) phosphorylated at four different sites (T212, T217, T231, and S235), contrary to unphosphorylated Tau, was unable to compete with the intramolecular interaction of BIN1 SH3 domain with its CLAP domain. In accordance, the affinity of BIN1 SH3 for phosphorylated Tau (210–240) peptide was reduced, with a five-fold increase in the dissociation constant, from a Kd of 44 to 256 μM. This study highlights the complexity of the regulation of BIN1 isoform 1 with Tau. As abnormal phosphorylation of Tau is linked to the pathology development, this regulation by phosphorylation might have important functional consequences.
Highlights
Alzheimer’s disease (AD) is the most common form of dementia and the most prevalent tauopathy
Based on the nuclear magnetic resonance (NMR) data, we proposed a structural model of the complex between Bridging integrator-1 (BIN1) SH3 and a Tau peptide, which provides the atomic detail of the interaction
We previously showed that BIN1 SH3 domain was engaged in an intramolecular interaction with BIN1 isoform 1 own clathrin and AP-2-binding domain (CLAP) domain (Malki et al, 2017)
Summary
Alzheimer’s disease (AD) is the most common form of dementia and the most prevalent tauopathy. Several hypothesis have emerged on how BIN1 protein contributes to the increased risk of developing AD, which could involve both Aβ and Tau neurodegeneration pathways (Chapuis et al, 2013; Calafate et al, 2016; Ubelmann et al, 2017). All BIN1 isoforms contain a N-terminal Bin/amphiphysin/Rvs (BAR) domain and a C-terminal SH3 domain (BIN1 SH3) (Prokic et al, 2014). Regulation of endosomal trafficking by BIN1 is proposed to affect Aβ42 endocytic production by negatively regulating APP and BACE1 convergence in early endosomes (Ubelmann et al, 2017). BIN1 loss of function prevents APP and BACE1 segregation at early endosomes and may boost Aβ accumulation
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have