Abstract
Integration of the human immunodeficiency virus type 1 (HIV-1) cDNA into the human genome is catalyzed by the viral integrase protein that requires the lens epithelium-derived growth factor (LEDGF), a cellular transcriptional coactivator. In the presence of LEDGF, integrase forms a stable complex in vitro and importantly becomes soluble by contrast with integrase alone which aggregates and precipitates. Using cryo-electron microscopy (EM) and single-particle reconstruction, we obtained three-dimensional structures of the wild type full length integrase-LEDGF complex with and without DNA [1]. The stoichiometry of the complex was found to be (integrase)4-(LEDGF)2 by mass spectrometry analysis and existing atomic structures were unambiguous positioned in the EM map. In vitro functional assays reveal that LEDGF increases integrase activity likely in maintaining a stable and functional integrase structure. DNA-Protein cross-linking experiments show specific interaction between viral DNA and the C-terminal domain of integrase. Upon DNA binding, IN undergoes large conformational changes. Cryo-EM structure underlines the path of viral and target DNA and a model for DNA integration in human DNA is proposed (see fig. fig.1,1, overleaf). Figure 1 Proposed mechanism for thei ntegration of viral cDNA into the host genome: The LEDGF envelope is represented in blue; the integrase tetramer is shown as atomic structures. The viral DNA is in orange and the target DNA in red. On target DNA binding, there ...
Highlights
Fabrice Michel1, Sylvia Eiler1, Florence Granger1, Jean-François Mouscadet2, Marina Gottikh3, Alexis Nazabal4, Stéphane Emiliani5, Richard Benarous6, Dino Moras1, Patrick Schultz1 and Marc Ruff*1
Frontiers of Retrovirology: Complex retroviruses, retroelements and their hosts Meeting abstracts - A single PDF containing all abstracts in this Supplement is available here. http://www.biomedcentral.com/content/pdf/1742-4690-6-S2-info.pdf
Integration of the human immunodeficiency virus type 1 (HIV-1) cDNA into the human genome is catalyzed by the viral integrase protein that requires the lens epitheliumderived growth factor (LEDGF), a cellular transcriptional coactivator
Summary
Fabrice Michel1, Sylvia Eiler1, Florence Granger1, Jean-François Mouscadet2, Marina Gottikh3, Alexis Nazabal4, Stéphane Emiliani5, Richard Benarous6, Dino Moras1, Patrick Schultz1 and Marc Ruff*1. Address: 1IGBMC, UDS, U596 Inserm, UMR7104 CNRS, 67404 Illkirch; France, 2Laboratoire de Biotechnologie et Pharmacologie Génétique Appliquée, CNRS, UMR8113, ENS-Cachan, 94235 Cachan, France, 3Belozersky Institute of Physico-Chemical Biology, Moscow State University, 119992 Moscow, Russia, 4CovalX, Technoparkstrasse, 1, CH-8005, Zürich, Zwitzerland, 5Institut Cochin, Université Paris Descartes, CNRS (UMR8104), Inserm, U567, Paris, France and 6CellVir SAS, Evry, France; Hybrigenics SA, Paris, France * Corresponding author from Frontiers of Retrovirology: Complex retroviruses, retroelements and their hosts Montpellier, France.
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