Structural Basis for Growth Differentiation Factor 5 (GDF5) Signaling Inhibition by Repulsive Guidance Molecules (RGMs)

Tomas Malinauskas,Benjamin Bishop,Thomas D Mueller,Christian Siebold

doi:10.1016/j.bpj.2017.11.2561

Tomas Malinauskas, Benjamin Bishop + Show 2 more

Open Access

https://doi.org/10.1016/j.bpj.2017.11.2561

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Abstract

Structural Basis for Growth Differentiation Factor 5 (GDF5) Signaling Inhibition by Repulsive Guidance Molecules (RGMs)

Highlights

2302-Pos Board B318 Cell Surface Calreticulin-LRP1 Binding and its Role in Apoptotic Cell Engulfment Romone M
It has been previously reported that the spatial organization of Eph receptors and their ligands at the cell-cell contact interface modulate signaling cascades in breast cancer cell lines, impacting tumor aggressiveness
It has been suggested that the formation of clusters of Eph receptors on the membrane correlates with an invasive phenotype

Summary

Introduction

2302-Pos Board B318 Cell Surface Calreticulin-LRP1 Binding and its Role in Apoptotic Cell Engulfment Romone M. Using genetically encoded FRET-based biosensors targeted to the bulk cytosolic compartment as well as lipid raft and non-raft membrane domains, we monitored changes in cAMP activity in response to bAR and EPR stimulation in HASM cells overexpressing (OE) AC2 or AC6. OE AC6 selectively inhibited cAMP responses to bAR stimulation in lipid raft-associated locations, while having no effect on cAMP produced by EPRs anywhere.

Results

Conclusion