Abstract
We sequenced and annotated the first complete mitochondrial genome (mitogenome) of Ledra auditura (Hemiptera: Cicadellidae: Ledrinae) and reconstructed phylogenetic relationships among 47 species (including 2 outgroup species) on the basis of 3 datasets using maximum likelihood (ML) and Bayesian inference (BI) analyses. The complete L. auditura mitogenome (length, 16,094 bp) comprises 37 genes [13 protein-coding genes (PCGs), 22 tRNAs, and 2 rRNAs], 1 control region, and 2 long non-coding regions. The first long non-coding region (length, 211 bp) is located between tRNA-I and tRNA-Q and the second region (length, 994 bp) between tRNA-S2 and ND1. All PCGs show ATN (Met/Ile) as their start codon and TAR as their stop codon. Except tRNA-S1 (AGN), which lacks the dihydrouridine arm, all tRNAs can fold into the typical cloverleaf secondary structure. The complete L. auditura mitogenome shows a base composition bias of 76.3% A + T (A = 29.9%, T = 46.4%, G = 13.3%, and C = 10.5%), negative AT skew of −0.22, and positive GC skew of 0.12. In ML and BI analyses, L. auditura was clustered with Evacanthus heimianus (Hemiptera: Cicadellidae: Evacanthinae) with strong branch support.
Highlights
We sequenced and annotated the first complete mitochondrial genome of Ledra auditura (Hemiptera: Cicadellidae: Ledrinae) and reconstructed phylogenetic relationships among 47 species on the basis of 3 datasets using maximum likelihood (ML) and Bayesian inference (BI) analyses
The mitogenome is 16,094-bp long, ranging between 15,131 bp of Ttocnadella arisana to 16,811 bp of Parocerus laurifoliae. Such variations in mitogenome length can be mainly attributed to difference in control region length[25]
Consistent with previous observations in Cicadellidae, the sequences of L. auditura mitogenome were highly conserved in terms of gene content, gene size, gene order, base composition, codon usage of protein-coding genes (PCGs), and RNA secondary structures
Summary
We sequenced and annotated the first complete mitochondrial genome (mitogenome) of Ledra auditura (Hemiptera: Cicadellidae: Ledrinae) and reconstructed phylogenetic relationships among 47 species (including 2 outgroup species) on the basis of 3 datasets using maximum likelihood (ML) and Bayesian inference (BI) analyses. Ledrinae is a relatively small subfamily within the large and diverse leafhopper family Cicadellidae, which comprises approximately 300 described species of 38 genera divided into 5 tribes. L. auditura is the first Ledrinae species whose phylogenetic relationships with 40 other leafhoppers and 5 treehoppers have been assessed using maximum likelihood (ML) and Bayesian inference (BI) analyses based on mitogenomes, thereby providing a basis for further molecular research on the related taxa
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