Structural and mechanical multi-scale characterization of white New-Zealand rabbit Achilles tendon

Abstract

Multi-scale characterization of structures and mechanical behavior of biological tissues are of huge importance in order to evaluate the quality of a biological tissue and/or to provide bio-inspired scaffold for functional tissue engineering. Indeed, the more information on main biological tissue structures we get, the more relevant we will be to design new functional prostheses for regenerative medicine or to accurately evaluate tissues. From this perspective, we have investigated the structures and their mechanical properties from nanoscopic to macroscopic scale of fresh ex-vivo white New-Zealand rabbit Achilles tendon using second harmonic generation (SHG) microscopy, atomic force microscopy (AFM) and tensile tests to provide a “simple” model whose parameters are relevant of its micro or nano structure. Thus, collagen fiber's crimping was identified then measured from SHG images as a plane sine wave with 28.4±5.8μm of amplitude and 141±41μm of wavelength. Young's moduli of fibrils (3.0GPa) and amorphous phases (223MPa) were obtained using TH-AFM. From these investigations, a non-linear Zener model linking a statistical Weibull's distribution of taut fibers under traction to crimp fibers were developed. This model showed that for small strain (<0.1), the amorphous inter-fibrils phase in collagen fibers is more solicited than collagen fibrils themselves. The results open the way to modeled macroscopic mechanical behavior of aligned-crimped collagen soft tissues using multi-scale tendon observations under static or dynamic solicitations.