Abstract
The effects were assessed by combining physiological experiments with morphological examination, using light and electron microscopy. Continuous, parasympathetic nerve stimulation, at frequencies varying from 1 to 10 Hz, caused a copious flow of saliva. Both acinar and granular tubule cells showed extensive degranulation, but the effects on tubule cells were the more dramatic. After stimulation, there was a marked loss of acidic mucosubstances from the acinar cells and an almost complete loss of neutral mucosubstances from the granular tubule cells; this was particularly evident with stimulation at higher frequencies (6–10 Hz).
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