Structural and functional renovation of urinary bladders after amniotic membrane implantation in dogs

Abstract

Amniotic membrane as a biocompatible and permi-selective immune barrier was harvested from full-term pregnant bitches and cryopreserved by using Dulbecco’s modified Eagle’s medium (DMEM) or Minimal essential medium (MEM) 50% medium in glycerol 50% at temperature below freezing −80°C. A defined 3cm×3cm patch in the craniodorsal surface of the bladder was excised and then autografted in 15 apparent healthy mongrel dogs. In the place of excised patches of 27 apparent healthy mongrel dogs, double layers of 4cm×5cm cryopreserved amniotic membranes were implanted. The results of plain and contrast radiography, ultrasonography, blood and urine analysis and histopathology confirmed that urinary bladder surgical defect that replaced by amniotic membrane implant, proved successful revival by normal structural, functional and contractile layers without any growth abnormalities throughout three months period. The successful implantation model of amniotic membrane will encourage its use on human beings for renovation in the surgical management of bladder cancers and restoring damaged or diseased urinary bladders.