Abstract
The structure of the charge separated state P+· Q-· reaction centres (RC’s) of Rhodobacter sphaeroides R-26 in which, the non-heme iron has been replaced by zinc (Zn-bRC’s) is studied using transient EPR spectroscopy. A qualitative description of the spin polarized transient EPR spectra of P+·Q-· is presented and used to draw conclusions about the relative orientations of P 865 +. and Q A -. . Experimental and calculated spectra are presented for fully deuterated and protonated Zn-bRC’s at three microwave frequencies: X-band (9 GHz), K-band (24 GHz) and W-band (95 GHz). The simulated spectra are calculated on the basis of independently determined experimental parameters. From the good agreement between the experimental spectra and those calculated using the ground state x-ray structure, it is concluded that no significant light induced structural changes occur. K-band spectra at 300 K and 50 K are compared and it is suggested that the narrowing of the spectrum at room temperature is a result of changes in the hyperfine couplings due to motion of the chromophores as well as a change in the charge distribution on (Math).
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