Abstract
We present the results of an analysis of the 16S rRNA-based taxonomical structure of bacteria together with an analysis of carbon source utilization ability using EcoPlate (Biolog, USA) metabolic fingerprinting assessment against the backdrop of physicochemical parameters in fifteen interconnected lakes. The lakes exhibit a wide spectrum of trophic gradients and undergo different intensities of anthropopressure. Sequences of V3–V4 16S rRNA genes binned by taxonomic assignment to family indicated that bacterial communities in the highly eutrophicated lakes were distinctly different from the bacterial communities in the meso-eutrophic lakes (ANOSIM r = 0.99, p = 0.0002) and were characterized by higher richness and more diverse taxonomical structure. Representatives of the Actinobacteria, Proteobacteria, Cyanobacteria, Planctomycetes, Verrucomicrobia, Bacteroides phyla predominated. In most cases their relative abundance was significantly correlated with lake trophic state. We found no similar clear relationship of community-level physiological profiling with lake trophic state. However, we found some significant links between the taxonomic and metabolic structure of the microbes in the studied lakes (Mantel’s correlation r = 0.22, p = 0.006). The carbon source utilization ability of the studied microorganisms was affected not only by the taxonomic groups present in the lakes but also by various characteristics like a high PO43− concentration inhibiting the utilization of phosphorylated carbon.
Highlights
Rapid anthropogenic eutrophication of natural freshwater environments has been an increasing problem in the last few decades[1]
We found that higher TSI values were related to bacterial (LB) and heterotrophic nanoflagellate (HNF) abundances (Fig. 1b)
We found a significant positive correlation between mean TSI values and the relative abundance of the phyla Planctomycetes, Verrucomicrobia, Cyanobacteria and Chloroflexi and a significant negative correlation between TSI and microorganisms belonging to the phyla Actinobacteria and Bacteroides
Summary
Rapid anthropogenic eutrophication of natural freshwater environments has been an increasing problem in the last few decades[1]. The eutrophic lake showed a larger representation of Flavobacteriia and Gammaproteobacteria, but it was impossible to create a clear picture of the relationships between microorganism phylogeny and physiology Understanding these complex relationships and the way they are influenced by human activity disturbing the physicochemical conditions of reservoirs and their trophic status is crucial for understanding the changes in biochemical cycles as a consequence of anthropopressure. We present an analysis of the community-level physiological profiling and phylogenetic diversity of the microbial community in 15 interconnected lakes located in the temperate climate zone of north-eastern Poland These lakes belong to the Great Masurian Lakes System (GMLS), which was formed by ice sheets during the Pomeranian phase of the glaciation in the late Pleistocene[20].
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