Abstract

The water-soluble extracellular polysaccharide produced by Asaia bogorensis, an acetic acid bacterium, was investigated. It was suggested that the water-soluble polysaccharide seemed to be a fructan as only fructose was detected as a constituent sugar when it was hydrolyzed by trifluoroacetic acid. To confirm the glycosidic linkage of the fructan, 1H NMR spectroscopy and 13C NMR spectroscopy were carried out. From these results, Asaia fructan was identified as levan, which is a β-2,6 linked fructan, similar to the polysaccharide produced by Gluconacetobacter xylinus. This β-2,6-linked levan-type fructan was produced only when sucrose was used as a carbon source.

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