Abstract
A methyl-esterified rhamnogalacturonan with neutral carbohydrate side-chains (PG-1), derived from an acidic pectic polysaccharide (AR-4IIc) from a hot-water extract of Angelica acutiloba Kitagawa, was subjected to base-catalysed β-elimination in the presence of sodium borodeuteride. The neutral carbohydrate chains, thereby released from position 4 of GalA residues and isolated by anion-exchange chromatography, were shown by gel-filtration chromatography and methylation analysis to consist of a high-molecular-weight galactan with some arabinosyl side-chains and material of lower molecular weight which consisted mainly of branched arabinosyl chains. Partial hydrolysis of PG-1 with acid gave a rhamnogalacturonan (MPG-1) possessing short oligosaccharide chains. β-Eliminative degradation of MPG-1 released neutral oligosaccharide chains terminated by galactitol- 1-d and rhamnitol- 1-d moieties. G.l.c.-m.s. showed that the neutral carbohydrates released from MPG-1 included Gal-(1→4)-Rha-ol- 1-d, Gal-(1→4)- Gal-ol- 1-d, Gal-(1→3)-Gal-ol- 1-d, Gal-(1→6)-Gal-ol- 1-d, Ara→Rha→Rha-ol- 1-d, and Gal→Gal→Gal-ol- 1-d.
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