Structural Analysis of Hypothetical Proteins of Salmonella Typhi CT18

Abstract

Background: Salmonella typhi is a gram negative, flagellated, rod shaped facultative, anaerobic bacterium belonging to family enterobacteriace. It is etiological agent of typhoid fever. It spreads through contamination of water and under cooked food. It has caused death in many countries where sanitation is poor. It has evolved the ability to spread in deeper tissues of human body..Objective: To identify the function and structure of hypothetical proteins of Salmonella typhi CT18 by using bioinformatic programs.Material and Methods: The 3D structure of these proteins was carried out by the help of Bioinformatics programs such as CMR, Interproscan, BLAST, Modellor 9.10, Procheck and Prosa. Functional annotation was carried out by using Interproscan and structure analysis was carried by homology modeling using Modeller.Results: It was found that there are 4934 proteins are present in Salmonella typhi out of which, 12.1 % were hypothetical proteins. Among hypothetical proteins, results of 463 proteins were shown by interproscan,6.3% were functionally detected proteins, 2.6% were uncharacterized proteins and for rest no hits were found. For homology modeling, 5 proteins were selected. The three dimensional structure of proteins was constructed by Modeller 9.10.Conclusion: Structural bioinformatics is an effective method to find structure of hypothetical protein which provide good solution for drug discovery.Keywords: Homology Modelling, Hypothetical proteins, Typhoid fever,Modellor, Structural Bioinformatics.