Abstract
BackgroundHCV infection is a major health problem causing acute and chronic hepatitis. HCV E1 protein is a transmembrane protein that is involved in viral attachment and therefore, can serve as an important target for vaccine development. Consequently, this study was designed to analyze the HCV E1 protein sequence isolated in Pakistan to find potential conserved epitopes/antigenic determinants.ResultsHCV E1 protein isolated in Pakistan was analyzed using various bio-informatics and immuno-informatics tools including sequence and structure tools. A total of four antigenic B cell epitopes, 5 MHC class I binding peptides and 5 MHC class II binding peptides were predicted. Best designed epitopes were subjected to conservation analyses with other countries.ConclusionThe study was conducted to predict antigenic determinants/epitopes of HCV E1 protein of genotype 3a along with the 3D protein modeling. The study revealed potential B-cell and T-cell epitopes that can raise the desired immune response against HCV E1 protein isolated in Pakistan. Conservation analysis can be helpful in developing effective vaccines against HCV and thus limiting threats of HCV infection in Pakistan.
Highlights
Hepatitis C Virus infection is a global health problem affecting 270 million people worldwide [1]
Bioinformatics analysis has opened new vistas to provide more insights into protein sequence and structural features. Both B-cell and T-cell epitopes/antigenic determinants are important in raising desired immune responses and the number of epitopes and modulation of immune recognition of antigens can be influenced by deglycosylation of viral glycoproteins [14]
Structural description of the model The present study was initiated to perform structure based sequence analysis studies on the HCV envelope protein 1 (E1) protein isolated in Pakistan
Summary
Hepatitis C Virus infection is a global health problem affecting 270 million people worldwide [1]. Bioinformatics analysis has opened new vistas to provide more insights into protein sequence and structural features. Both B-cell and T-cell epitopes/antigenic determinants are important in raising desired immune responses and the number of epitopes and modulation of immune recognition of antigens can be influenced by deglycosylation of viral glycoproteins [14]. This study was designed to perform immunoinformatic analysis on the HCV E1 glycoprotein isolated in Pakistan and to analyze antigenicity, hydrophobicity, surface accessibility and epitopic location of epitopes in HCV glycoprotein structure. HCV infection is a major health problem causing acute and chronic hepatitis. This study was designed to analyze the HCV E1 protein sequence isolated in Pakistan to find potential conserved epitopes/antigenic determinants
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