Structural analyses of the lipopolysaccharides from Chlamydophila psittaci strain 6BC and Chlamydophila pneumoniae strain Kajaani 6

Abstract

Lipopolysaccharides (LPSs) of Chlamydophila psittaci 6BC and Chlamydophila pneumoniae Kajaani 6 contain 3-deoxy- d- manno-oct-2-ulosonic acid (Kdo), GlcN, organic bound phosphate, and fatty acids in the molar ratios of ∼3:2:2.2:4.8 and ∼2.9:2:2.1:4.9, respectively. The LPSs were immunoreactive with a monoclonal antibody against a family-specific epitope of chlamydial LPS. This finding, together with methylation analyses of both LPSs and MALDI-TOF MS experiments on de- O-, and de- O, N-acylated LPSs, indicate the presence of a Kdo trisaccharide proximal to lipid A having a structure α-Kdo-(2→8)-α-Kdo-(2→4)-α-Kdo, which appears to be the main component of the core region in the native chlamydial LPSs. In the de- O-acylated LPSs from Chl. psittaci 6BC and Chl. pneumoniae Kajaani 6, two major molecular species are present that differ in distribution of amide-bound hydroxy fatty acids over both GlcN. It appears that either two ( R)-3-hydroxy-18-methylicosanoic acids or one ( R)-3-hydroxy-18-methylicosanoic acid and one ( R)-3-hydroxyicosanoic acid are attached to the GlcN residues. In contrast, the de- O-acylated LPS of Chl. psittaci PK 5082 contains one major molecular species that has two ( R)-3-hydroxyicosanoic acid residues attached to two GlcN residues.