Strontium ranelate inhibits alveolar bone resorption in diabetic rats with periodontitis by regulating JAK2/STAT3 signaling pathway

Abstract

Purpose: To investigate the inhibitory effect of strontium ranelate on alveolar bone resorption in diabetic rats with periodontitis, and the mechanism involved.Methods: Forty-five Sprague-Dawley (SD) rats were allocated to 3 groups, viz, diabetic control, diabetic periodontitis, and diabetic periodontitis + strontium ranelate groups. Diabetes was induced via i.v. administration of streptozotocin (STZ) (45 mg/kg). Then, a rat model of periodontitis was established by ligating the neck of the upper left first molar of each rat with a 0.4-mm orthodontic ligation wire. Strontium ranelate or normal saline was administered by gavage. Four weeks later, various indicators were assessed.Results: There was a significantly higher expression level of Mir-21 in the periodontal tissue of diabetic periodontitis rats than in diabetic control rats. In contrast, this parameter was significantly lower in diabetic periodontitis rats than in periodontal tissues of rats in the diabetic periodontitis + strontium ranelate group, while the translations of JAK2 and STAT3 genes were significantly higher in periodontal tissues of diabetic periodontitis rats (p < 0.05). In contrast, there were lower expression levels of JAK2 and STAT3 in diabetic periodontitis group than in diabetic periodontitis + strontium ranelate group (p < 0.05).Conclusion: Strontium ranelate increases the expression level of miR-21 and activates JAK2/STAT3 signaling pathway, thereby inhibiting the resorption of alveolus bone in rats with diabetes/periodontitis.