Abstract
Hydroxyapatite can deliver drugs, and its composite material is capable of repairing bone defects in tumors. This study was conducted to evaluate the effect of composite materials on tumor growth inhibition and bone growth induction. Composites containing drug delivery compounds were synthesized by coprecipitation and freeze-drying and then characterized by scanning electron microscopy (SEM), X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy (FTIR). In addition, the effect of hydroxyapatite nanoparticles (nano-SHAP) on proliferation of an osteosarcoma cell line (MG-63) and an osteoblast cell line (MC3T3-E1) was evaluated, and its mechanism was studied. The use of nano-SHAP alone did not affect the proliferation of normal cell lines. However, nanoparticles containing different amounts of norcantharidin in the composite materials and had different inhibitory effects on osteosarcoma and different effects on osteoblasts. And, with the increase of the content of norcantharidin, the antitumor performance of the composite has been enhanced. In summary, the nano-SHAP system developed in this study is a drug delivery material that can inhibit the growth of tumors and induce the proliferation of osteoblasts.
Highlights
Osteosarcoma (OS) is the most common primary malignant solid tumor in humans between the ages of 10 and 25 [1]
After stirring and mixing smoothly, 30 g KH2PO4 was added slowly to keep the ratio of (Ca + Sr)/P at 1.67 and pH at 10, which was mixed for 24 hours. e above two solutions were mixed and divided into four parts, adding 0 g, 5/6 g, 5/3 g, and 5/2 g norcantharidin, respectively. e above liquids were fully mixed and added to 24-hole plate which was freeze-dried overnight in a freeze-dryer at − 60°C. e mixtures were marked as Strontium/chitosan/hydroxyapatite/norcantharidin0, Strontium/chitosan/hydroxyapatite/norcantharidin1, Strontium/chitosan/hydroxyapatite/norcantharidin2, and Strontium/chitosan/hydroxyapatite/norcantharidin3
Figures 1(a)− 1(d) shows the morphology of the synthesized nanoparticles. e scanning electron microscopy (SEM) images show that the materials were porous, that the pore size of the Q1, Q2, and Q3 composite materials was smaller than that of Q0, that the materials were made of small needle-like or plate-like crystals, and that the structure changed with the addition of NCTD. e materials had an irregular three-dimensional (3D) structure, and their pores were tightly connected to each other. e 3D environment may enhance cell-cell interaction and promote cell biological activity [33, 34]. us, the prepared material may be suitable for bone repair
Summary
Osteosarcoma (OS) is the most common primary malignant solid tumor in humans between the ages of 10 and 25 [1]. It is characterized mainly by the destruction of and recurrence in local tissue and is prone to distant metastasis. Tumor cells can remain in the area around the tumor resection site after surgery, resulting in high recurrence and bone destruction. Adjuvant chemotherapy plays an important role in killing the residual tumor cells and preventing tumor metastasis and recurrence [3]. Allogeneic bone is one of the most commonly used materials used for repairing tumor-induced bone defects, but it has no antitumor effect and is associated with the spread of infectious diseases and immune response [4]. Allogeneic bone is one of the most commonly used materials used for repairing tumor-induced bone defects, but it has no antitumor effect and is associated with the spread of infectious diseases and immune response [4]. erefore, biocompatible biomaterials have the potential to be anticancer agents and to repair defects
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
