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Abstract
This study aimed to evaluate the effects of the stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs) on cartilage injury in an osteoarthritis (OA) rat model. Sodium iodoacetate (3 mg/50 μL) was used to induce OA in the left knee joint of rats. On day 14 after OA induction, 50 μL of SVF (5 × 106cells), ADSCs (1 × 106 cells), or 0.9% normal saline (NS) was injected into the left knee-joint cavity of each group. The macroscopic view and histological sections revealed that the articular cartilage in the NS group was damaged, inflamed, uneven and thin, and had hyperchromatic cell infiltration. Notably, the cartilage surface had recovered to nearly normal and appeared smooth and bright on day 14 in the SVF and ADSC groups. Additionally, the white blood cell counts in the SVF and ADSC groups were higher than those in the NS group on day 14. Plasma IL-1β levels on days 7 and 14 were reduced in the SVF and ADSC groups. These results indicated that both SVF and ADSC treatments may assist in articular cartilage regeneration after cartilage injury. Cell therapy may benefit patients with OA. However, clinical trials with humans are required before the application of SVF and ADSC treatments in patients with OA.
Highlights
This study aimed to evaluate the effects of the stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs) on cartilage injury in an osteoarthritis (OA) rat model
No obvious appearance of OA was observed through the cartilages of the patellar surface, lateral and medial condyles, and tibial plateau in the SVF and ADSC treatment groups, which recovered similar to the normal articular cartilage (Fig. 2). These results suggested that SVF and ADSC treatments may contribute to cartilage repair and regeneration in OA
On days 7 and 14 after SVF/ ADSC treatment, a visible thick layer of cartilage without irregular surface was observed, which was recovered similar to that of the normal cartilage (Fig. 3, indicated with red bars). These results suggested that both SVF and ADSC treatments may contribute to cartilage regeneration in OA
Summary
This study aimed to evaluate the effects of the stromal vascular fraction (SVF) and adipose-derived stem cells (ADSCs) on cartilage injury in an osteoarthritis (OA) rat model. Plasma IL-1β levels on days 7 and 14 were reduced in the SVF and ADSC groups. These results indicated that both SVF and ADSC treatments may assist in articular cartilage regeneration after cartilage injury. A rat model of sodium iodoacetate-induced OA was employed to compare the tissue repair effects of intra-articular injections of the SVF and ADSCs. chondrocytes under OA stress metabolically activate and typically increase the gene expression of several matrix components (including Col-2) to restore the extracellular matrix[13–15]. Collagen synthesis was analyzed by examining the pathological slides of each group
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