Abstract
Strigolactones regulate root development and are known to inhibit adventitious root formation. However, the hormonal targets of strigolactones during adventitious rooting are yet to be determined. As a signal molecule, H2O2 stimulates adventitious rooting. On the other hand, plasma membrane H+-ATPase is involved in several hormonal signals that are linked to root development. This study evaluated the influence of a synthetic strigolactone (rac-GR24), H2O2, and plasma membrane H+-ATPase on adventitious root formation in mung bean hypocotyls. We also determined if H2O2 and plasma membrane H+-ATPases are possible hormonal targets in strigolactone-mediated inhibition of adventitious rooting. The results confirm the inhibitory role of strigolactones and the enhancing influence of H2O2 on adventitious rooting while also suggesting that plasma membrane H+-ATPase activity is necessary and may be employed to regulate adventitious rooting. Also, rac-GR24 diminished endogenous H2O2 content by inhibiting NOX and SOD activities while also inhibiting plasma membrane H+-ATPase activity. Therefore, we conclude that interference with H2O2 signaling and plasma membrane H+-ATPase activity may serve as a potent hormonal mechanism that may be employed by strigolactones to downregulate adventitious rooting in mung bean hypocotyls.
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